CLOSTRIDIUM DIFFICILE MANNITOL TAUROCHOLATE BROTH BASE

Clostridium difficile Mannitol Taurocholate Broth Base is used for the primary isolation of C. difficile from faecal specimens. The spectrum of disease caused by Clostridium difficile (a pathogenic Clostridium affecting the bowel) ranges from pseudomembranous colitis (PMC) through antibiotic associated colitis (AAC). It also includes chronic inflammatory bowel diseases, post-operative diarrhoea and non-antibiotic associated diarrhoea. Smith and King first reported the presence of C. difficile in human infections. The medium composition is designed so as to obtain luxuriant growth of C. difficile. The selective agents D-cycloserine and cefoxitin used in the medium inhibit the growth of majority of Enterobacteriaceae and also Enterococcus faecalis, gram-negative anaerobic bacilli and Clostridium species other than C. difficile, which may be found abundantly in faecal samples.

for cultivation of Clostridium di?cile from certain clinical specimens