COLUMBIA BLOOD AGAR BASE (VEG.)

These media are prepared by using Veg special peptone which is free from BSE/TSE risks. Columbia Blood Agar Base/ w/ 1% Agar Veg is the modification of Columbia Blood Agar Base/ w/ 1% Agar which was originally developed by Ellner et al. The media promotes typical colonial morphology, better pigment production and more sharply defined haemolytic activity. Columbia Blood Agar Base/ w/ 1% Agar. Veg is used as a base for preparing media containing blood and for selective media formulations in which different combinations of antimicrobial agents are used as additives. Sheep blood permits the detection of haemolysis and also provides heme (X factor) which is required for the growth of many fastidious bacteria. However, it is devoid of V factor (Nicotinamide adenine dinuellotide) and hence Haemophillus influenzae which needs both X and V factors, will not grow on this media. As these media has a relatively high carbohydrate content betahaemolytic Streptococci may exhibit a greenish haemolytic reaction which may be mistaken for alpha haemolysis therefore confirmatory tests for all the colonies should be carried out.

for preparation of various selective & identi?cation media & isolation of organisms from clinical specimens