CPC AGAR BASE W/ 1% CELLOBIOSE

Vibrio species are natural inhabitants of brackish and salt water. Human disease is associated with ingestion of contaminated water or consumption of contaminated seafood. Wound and systemic infections develop following contact with contaminated water. CPC (Cellobiose, Polymyxin and Colistin) Agar Base w/1% cellobiose, is formulated in accordance with FDA BAM for the differentiation of Vibrio vulnificus from other Vibrio’s. Vibrio cholerae strains except V.cholerae 01-classical biotype grow on CPC Agar while most Vibrio parahaemolyticus strains do not grow on CPC Agar. If growth occurs, colonies appear green purple coloured due to lack of cellobiose fermentation.
Blend approximately 25 grams of food sample with 225 ml Alkaline Peptone Water and incubate at 35 ?2?C for 6 to 8 hrs to overnight depending on the sample. Transfer a loopful culture from this to the surface of the dried plates of CPC Agar Base w/1% cellobiose with Modified CPC Supplement for mCPC Agar or Colistin Supplement for CC Agar; incubate at 39 – 40?C for 18 to 24 hours. Typical colonies of V. cholerae are small, smooth, opaque and green to purple in colour as the medium contains two pH indicators viz. bromothymol blue and cresol red. A purple background will also be developed upon extended incubation. Biochemical tests are performed to confirm the organisms.

for the cultivation and identi?cation of Vibrio species from foods in accordance with FDA BAM, 1998