CRYSTAL VIOLET LACTOSE AGAR

Crystal Violet Lactose Agar was recommended by Chapman for the differentiation of pure cultures of pathogenic from nonpathogenic strains of Staphylococci. The toxicity of Staphylococci is estimated on the basis of their pigment production, haemolytic and coagulating characteristic. In the study of the correlation between haemolytic and coagulase activities, animal inoculation and other tests, Chapman and Berens reported that Staphylococci produced different coloured growths when cultured on Crystal Violet Agar. Haemolytic and coagulating strains produced purple to violet colour whereas non-hemolytic and non-coagulating strains produced white colonies after incubation. Crystal violet inhibits most of the gram-positive organisms and is markedly inhibitory to Staphylococci. A fair growth can be obtained at a 1: 300,000 concentration of the dye when the medium is inoculated heavily. So, this medium is used for study of pure cultures where a mass inoculation can be used rather than for primary isolation.

for di?erentiation of pure cultures of pathogenic and nonpathogenic Staphylococci