CULTURE MEDIUM FOR RWC (DISINFECTANT TEST BROTH) (RWC MEDIUM) (as per BIS)

Rideal and Walker developed phenol coefficient test in 1903 for determining the germicidal efficiency of disinfectants. In addition to being a satisfactory index of the germicidal value of phenol like disinfectants, the phenol coefficient is used as basis for determining the dilutions, which may safely be employed in practice. The phenol coefficient of each disinfectant was first determined by the Food and Drug Administration method, 1931. Since then, there is no standard method for testing disinfectants under practical conditions. Culture Medium for RWC is used for testing disinfectants and especially for determining phenol coefficient of disinfectants using Salmonella Typhi.

for determination of phenol coe?cients of disinfectants using Salmonella Typhi as a test organism