D.C.L.S. AGAR, HAJNA

Salmonella infection leads to salmonellosis, which ranges clinically from self-limited gastroenteritis (diarrhea, abdominal cramps and fever) to enteric fevers (including typhoid fever). Shigella species causes classical bacillary dysentery characterized by severe cramping abdominal pain and diarrhea with blood and mucus. Deoxycholate Citrate Lactose Sucrose (DCLS) Agar was originally formulated by Leifson and further modified by Hajna and Damon. It is a moderately selective medium for the isolation of gram-negative enteric bacilli from faecal specimens. This medium supports the growth of Salmonella, Shigella species and aerobic Vibrio like Vibrio comma, while coliforms and Proteus are inhibited. Salmonella Pullorum and Salmonella Gallinarum grow well on this medium.

for isolation of gram negative enteric bacilli