DECARBOXYLASE BROTH BASE, MOELLER (MOELLER DECARBOXYLASE BROTH BASE) (VEG.)

These media are used for differentiating gram-negative enteric bacilli on the basis of their ability to decarboxylate amino acids. The Decarboxylase Broth was introduced by Moeller for detecting the production of lysine and ornithine decarboxylase and arginine dihydrolase. Prior to Moeller?s work, bacterial amino acid decarboxylases were studied by Gale and Gale and Epps. These Veg media are prepared by replacing animal based peptones with vegetable peptones which are BSE/TSE risks free. Production of ornithine decarboxylase is a helpful criterion in differentiating Klebsiella and Enterobacter species. Klebsiella are non-motile and do not produce ornithine decarboxylase while Enterobacter are motile and produce ornithine decarboxylase except Enterobacter agglomerans.

for di?erentiation of bacteria on the basis of their ability to decarboxlyate the amino acid