DEXTROSE BROTH (GLUCOSE BROTH)

Dextrose in culture media serves as a source of energy. Dextrose Broth is useful when the organism has to be revived from small innocula. Dextrose Broth can also be used for anaerobic growth by the addition of 0.1- 0.2% Agar. Agar, thus added, helps to disperse the growth formed and also expel the CO2 formed. Facultative aerobic organisms tend to grow near the surface, in upper zone of the tube. Dextrose Broth is used for antibiotic sensitivity testing using the tube dilution method. Sensitivity testing of neomycin and chlortetracycline is better done using this medium.

for antibiotic sensitivity testing by tube dilution method for the isolation, cultivation and enumeration of di?erent microorganisms