DIFFERENTIAL REINFORCED CLOSTRIDIAL BROTH BASE

Hirsch and Grinstead originally described Differential Reinforced Clostridial Medium to initiate the growth from small inoculum and get a higher Clostridial count. Later, Barnes and Ingram used the medium to develop vegetative cells in assays of Clostridium perfringens. This medium is developed for the isolation of sulphite-reducing Clostridia from food and for their enumeration in water by multiple tube method. Differential Reinforced Clostridial Broth is used to determine the count of sulphite reducing bacteria by MPN technique.

for cultivation of Clostridia from water