DNASE TEST AGAR W/ TOLUIDINE BLUE (VEG.)

This medium is prepared by using Veg Hydrolysate No.1 which is free from BSE/TSE risks. DNase Test Agar (Veg) is the modification of DNase Test Agar which is used for detecting deoxyribonuclease activity of bacteria and fungi and particularly for identification of pathogenic Staphylococci. With toluidine blue, it is used in differentiation and identification of nonpigmented Serratia species isolated from clinical sources that might be improperly identified as Enterobacter and Klebsiella species. DNase activity was observed by Weckman and Catlin in Micrococci and found the correlation with coagulase activity as coagulase positive species were DNase positive. Di Salvo confirmed the results of Weckman and Catlin and observed accurate correlation of DNase and coagulase activity. In his experiment Di Salvo incorporated DNA and calcium chloride to activate DNase enzyme. DNase medium was modified by adding toluidine blue by Schreier. Modified medium achieved faster identification of Serratia marcescens and could differentiate Serratia from other members of the Enterobacteriaceae.

for detection of deoxyribonuclease activity of microorganisms and for identi?cation of staphylococci