ESCULIN AZIDE BROTH

Esculin Azide Broth is prepared as per the modification of original formula of Isenberg. Enterococci are able to hydrolyze esculin whereas other streptococci are not able to do so. Swan used an esculin medium containing 40% bile salts and reported that a positive reaction on bile esculin medium could be correlated with a serological group D precipitin reaction. Further studies by Facklam and Moody presumptively identified group D Streptococci and found that the bile esculin test provided a reliable means of identifying group D Streptococci and differentiating them from non-group D Streptococci. The present formulation is a modification of Bile Esculin Agar formulated by Isenberg et al in which bile concentration was 40 g/l.
In Esculin Azide Broth, the concentration of bile was reduced to 10 g/l and also additional sodium azide was added. The broth is selective due to presence of bile salts and sodium azide and provides rapid growth of Streptococci.

for selective cultivation and identi?cation of Streptococci