GELATIN AGAR

Members of the genus Vibrio are facultative anaerobes capable of both respiratory and fermentative metabolism. The natural habitat for Vibrio species is aquatic, in both fresh water and salt water. The growth and biochemical reactivity of most species are enhanced in different test media supplemented with 1- 2 % sodium chloride. Vibrios are fairly easy to isolate from both clinical and environmental material, though some species may require growth factors and /or vitamins. Media can be made selective for Vibrio’s by adding appropriate selective agents. High concentrations of NaCl and alkaline pH have also been used to select certain Vibrio species, based on the ability of most Vibrio’s to grow at pH values above 8.0 and at 3% or higher concentrations of NaCl. Gelatin Agar is formulated in accordance with APHA for the cultivation and characterization of Vibrio species from foods and faeces. Clinical specimens must be obtained early in the disease as possible because the duration of excretion of the pathogen is short.

for cultivation and identi?cation of Vibrio species