GLUCOSE BROTH W/ 0.05% SPS (BLOOD CULTURE BOTTLE)

Blood is one of the most important specimens received by the laboratory and blood culture is one of the most important and critical procedures performed in the microbiology laboratory. The isolation and identification of an organism has great diagnostic significance with blood being normally sterile. Blood cultures are of great importance in diagnosing such conditions as endocarditis, typhoid fever, pneumonia, supportive thrombophlebitis, infection of vascular grafts and other disease characterized by bacteremia.
Glucose Broth supplemented w/ 0.05% SPS support the growth of a wide variety of clinically important pathogenic microorganisms, including fastidious organisms. Waisbren, Carr and Dunnett used Glucose Broth for testing antibiotic sensitivity by the tube dilution method. This medium is also used to study glucose fermentation where pH indicator is not desired. Glucose Broth was developed to exclude the ingredients like beef extract that would contain small amount of carbohydrates. Thus, the glucose fermentation studies can be performed more accurately using only pure 0.5% glucose as the source of carbohydrate.