H BROTH

The genus Salmonella is a member of the family Enterobacteriaceae. Salmonella has three kinds of major antigen with diagnostic or identifying applications: Somatic (O) or Cell Wall Antigens, Surface (Envelope) Antigens and Flagellar (H) Antigens. Identification and differentiation of Salmonella based on H antigen were found to be less labor-intensive than the standard methods while requiring no more technical skill. H Broth, used for the preparation of H antigen in the identification and differentiation of Salmonella, was originally formulated by Hajna and Damon. The medium is also used for differentiating Enterobacteriaceae. The combination of Tryptone and Peptone makes the medium highly nutritive for the growth of gram-negative enteric bacteria.

for preparation of ?H? antigen, used in the identi?cation and di?erentiation of Salmonella species