SOB MEDIUM (HANAHAN’S BROTH)

Transformation is a process involving the uptake of foreign genetic material, which on subsequent recombination event results into genetically altered cell. The ability of a bacterium to take up exogenous DNA from the extracellular environment is termed as competence of the bacterium. Factors affecting the cell surface are important to competence, particularly changes in the membrane permeability, so as to allow the foreign DNA to enter the recipient cell. Bacteria undergoing transformation need to be cultured on a rich, isotonic medium to overcome or recover from the process of transformation by mending the perforations caused by transformation and undergo replication. Hanahans Broth developed by Hanahan is used for the cultivation of these recombinant Escherichia coli strains that have undergone transformation.

for cultivation of recombinant strains of Escherichia coli