KING’S MEDIUM B BASE

Pseudomonas aeruginosa is known to produce two types of pigments, pyocyanin and fluorescein which is a characteristic property and aids in isolation of Pseudomonas from clinical material. An additional pigment called as pyorubin was reported by King. Pyocyanin is green while fluorescein is fluorescent yellow and pyorubin is reddish brown. Some strains produce all these pigments while the others produce one or two pigments. P.aeruginosa can be identified on Hugh Leifson Medium. Kings Medium B Base is particularly suited for fluorescein.
Kings Medium B Base is based on the formulation of King et al. This medium can be used as a general medium for the non-selective isolation and pigment production of Pseudomonas species from foods, cosmetic samples etc. Agrobacterium have been traditionally identified as gram-negative bacteria that do not produce fluorescent pigment on Kings B medium and do produce tumors (or hairy roots) when inoculated onto test plants.

for non-selective isolation, cultivation and pigment production by Pseudomonas species