KOHN TWO TUBE MEDIUM NO. 2

Russell first introduced Double Sugar Medium, a differentiating medium for Enterobacteriaceae. Kohn later developed a technique employing two tubes of composite media for study of culture reactions, for the identification of Enterobacteriaceae. Gillies further made minor modifications in Kohns media. Kohn Two Tube Medium No.2 is used to study carbohydrate fermentation (Sucrose and Salicin) along with motility, hydrogen sulfide production and indole production.

for identi?cation of Enterobacteriaceae on the basis of sucrose and salicin fermentation, motility, hydrogen sulphide and indole production