L.D. EGG YOLK AGAR BASE

Organisms that grow in the absence of oxygen are termed as anaerobes. Depending upon their ability to tolerate oxygen, they are classified as either facultative or obligate anaerobes. The anaerobic gram-negative bacteria are part of the normal flora of the upper respiratory tract, mouth, intestinal tract and urinogenital tract of human and animals. The bile-resistant Bacteroides fragilis group is the most commonly recovered anaerobe in clinical specimens and is more resistant to antimicrobial agents than any other anaerobe. Fusobacterium necrophorum is a very virulent anaerobe that may cause severe infections, usually in children or young adults.
L. D. Medium or Lombard-Dowell Medium was developed by Dowell and Lombard for the cultivation and identification of fastidious anaerobic bacteria. L. D. Egg Yolk Agar Base, along with egg yolk emulsion is used for the detection of lipase; lecithinase and proteolytic activity of both spore forming and non-spore-forming obligate anaerobes.

for identi?cation of lecithinase activity by anaerobic microorganisms