LITMUS MILK (as per BIS)

Litmus Milk is the most useful medium in dairy industry as it is a reliable indicator of bacterial action on milk. Present formulation is recommended by BIS for detection of bacteria responsible for food poisoning. For detection of Clostridium perfringens in water, inoculate freshly heated tubes of Litmus Milk with various quantities of water and heat at 80?C for 10-15 minutes to destroy non-sporing organisms. Examine after every 24 hours for positive Stormy Clot reaction at 35?C up to 5 days. Anaerobiosis in Litmus Milk can be obtained by adding a small heated iron nail or 0.1 gram of reduced iron to the medium.

for determination and maintenance of Lactobacilli