LIVER INFUSION AGAR

Brucella, a gram-negative intracellular parasite causes epizootic abortions in animals and septicemic febrile illness or localized infection of bone, tissue or organ systems in humans. Brucella species are the causative agents of Brucellosis, a zoonotic disease with a domestic animal reservoir. Tryptose Agar with 5% serum remains the media of choice for isolation of Brucella species. However, the growth is highly enhanced when grown on Liver Infusion or Brucella Agar, due to the high nutritive content of the infusion media. Further enhancement of growth can be achieved by the addition of 5% horse or rabbit serum to the medium. While isolating Brucella species from samples such as contaminated milk, inhibition of accompanying gram-positive bacteria is attained by the addition of crystal violet. Half strength Liver Infusion Agar can be used for the isolation of Entamoeba histolytica.

for cultivation of Brucella and other pathogenic anaerobic bacteria