M-BRILLIANT GREEN BROTH

Brilliant green broth is differential selective medium, used for the primary screening of Salmonella in polluted water through membrane filter technique. Salmonella is a gram-negative, non-sporulation, facultative anaerobic, non-motile rod in the family Enterobacteriaceae. They are widely distributed in animals causing diseases mainly in stomach and the intestines. It is difficult to differentiate these organisms biochemically from Escherichia coli. Membrane screening technique developed by Geldreich and Jeter. Application of the M-Brilliant Green Broth for primary screening of Salmonella in polluted water was developed by Kabler and Clark. Modification of Brilliant Green Agar without agar in double strength is modified into this selective differential medium. Salmonella are unable to ferment either lactose or saccharose in the medium. This allows identification of accompanying weakly lactose-positive or lactose-negative, but saccharose positive microorganisms.

for the detection of Salmonellae by the membrane ?lter technique