M- ENRICHMENT BROTH

In microbiological analysis of water samples includes membrane filter technique which is an alternative to most probable number (MPN) method. In membrane filter technique, through organic membrane using a filter funnel and vacuum system the fluid sample is passed. The surface of the membrane is concentrated with an organism. The filter membrane is then placed on surface of nutrient agar plate. The colonies are counted to determine the number of bacteria originally present. In water and waste water bacteria may become stressed or injured. Due to structural or metabolic damage these injured bacteria cannot grow and don?t form colonies in standard conditions. Incubating and initially culturing in enriched, non-inhibitory medium is used to enhance the resuscitation of stressed or injured organisms M-Enrichment Broth is used for the preliminary enrichment of organism.
If small numbers of organisms are present that can be detected by enrichment. Clark et al described the formula of M-Enrichment Broth is prepared according to the formula described by. This medium is also recommended for use in conjunction with M-EMB Broth and M-Bismuth Sulphite Broth. Many authors have recommended this medium for enrichment of organisms prior to isolation .M-Enrichment Broth medium is devoid of any carbohydrate source or indicator. However, these ingredients (or may be other ingredients also) can be added to this nutritive medium that can obtain a variety of media which are capable of demonstrating biological characteristics of microorganisms.

for enumeration of bacteria by membrane ?lter technique and for preliminary enrichment of organisms on membrane ?lter prior to using selective media