M-FC BROTH BASE

M-FC Broth Base, designed by Geldreich et al is used for the detection and enumeration of faecal coliforms using the membrane filter technique. This medium is based on the property of faecal coliforms to grow at 44-45?C. M-FC Broth Base is recommended by APHA and by various other standards for detection of faecal coliforms. APHA recommends the membrane filtration procedure and delayed incubation for faecal coliforms.
Sterile cotton absorbent cotton pads are saturated with M-FC Broth Base by addition of 2 ml medium. Membrane filters, through which water sample is passed, are aseptically placed onto sterile cotton absorbent cotton pads saturated with M-FC Broth Base. If total coliforms are to be estimated, incubation is carried out at 35-37?C whereas if faecal coliform count is to be estimated, incubation is done at 44-45?C. Coliforms will form blue colonies whereas non-coliforms will form gray coloured colonies on M-FC Broth Base.

for detection and enumeration of faecal coliforms by membrane ?lter technique at higher temp. (44.5?C)