M-HD ENDO BROTH W/ BG

Coliform bacteria are traditionally described as a group of bacteria that are aerobic and facultative anaerobic, gram-negative, non-spore forming, rod-shaped, fermenting lactose with gas and acid production at 35?C in 24 to 48 hours. The bacteria are classically used as indicators of faecal contamination or water pollution from sewage and thus are of sanitary significance. Coliform bacteria usually originate from the intestinal tract of warm blooded animals and may also originate from waters from wood industry, surfaces of red wood water tanks, biofilms within drinking water distribution systems.
M-HD Endo Broth w/ BG is formulated as per Hajna and Damon and is used for detection of coliforms in highly polluted water using membrane filter technique. This medium is a modification of M-HD Endo Broth with the basic fuchsin in the later being replaced with Brilliant green in the former.
Sterile cotton absorbent pads are saturated with around 2 ml of M-HD Endo Broth w/ BG. Membrane filter through which the test water sample has been passed is aseptically placed on these cotton pads. Following an incubation at 35-37?C for 18-24 hours, lactose-fermenting coliforms will from green colonies with metallic sheen. Non-lactose fermenting coliforms will form colourless colonies.

for the detection of coliform in highly polluted waters using membrane ?lter technique