M-(HPC) HETEROTROPHIC PLATE COUNT BROTH BASE

Heterotrophs are organisms including bacteria, yeasts and moulds that require an external source of organic carbon for growth. The heterotrophic plate count (HPC), formerly known as the standard plate count, is a procedure for estimating the number of live heterotrophic bacteria in water and measuring changes during water treatment and distribution or in swimming pools. Heterotrophic Plate Count Method has been applied in many variants and is widely used to measure the heterotrophic microorganism population in drinking water and other media. Three different methods are described for determining the heterotrophic plate count i.e. pour plate method, spread plate method and membrane filter method. M-(HPC) Heterotrophic Plate Count Broth Base can also be employed for the determination of Heterotrophic Plate Count by the membrane filter method. Sterile cotton absorbent pads are saturated with the broth medium. Membrane filters are then placed on these saturated cotton absorbent pads and incubated.

for enumeration of heterotrophic microorganisms from water by membrane ?lter technique