M-LAURYL SULPHATE AGAR

Burman substituted bile salts with teepol in Membrane Enriched Teepol Broth, the membrane filtration test medium used to detect coliform organisms in water. M-Lauryl Sulphate Agar is prepared by substituting teepol with sodium lauryl sulphate.
The water samples are filtered through sterile membrane filter and then placed face upward on agar plates contains M-Lauryl Sulphate Agar. Burman recommended the following incubation temperatures and durations.
Unchlorinated waters:
Coliform organisms :4 hours at 30?C followed by 14 hours at 35?C
Escherichia coli: 4 hours at 30?C followed by 14 hours at 44?C
Non-chlorinated organisms benefit from 4 hours? incubation at 30?C but chlorinated organisms require 6 hours incubation at 25?C. After incubation, yellow colonies are formed which should be confirmed further.

for enumeration of Escherichia coli and coliforms in water, using membrane ?lter technique