M17 BROTH

M17 broth is based on the formulation described by Terzaghi and Sandine for the cultivation and enumeration of lactic Streptococci and their bacteriophages. M17 Broth is a modification of M16 Medium. Lactic Streptococci are nutritionally fastidious and require complex media for optimal growth. Disodium glycerophosphate maintains the pH above 5.7. The maintenance of pH is very important as the lower pH results in injury and reduced recovery of lactic Streptococci. Glycerophosphate does not form precipitate with calcium which is needed for the plaque assay of lactic bacteriophages.

for cultivation and isolation of lactic Streptococci and their bacteriophages