MICRO VITAMIN TEST CULTURE AGAR

Lactobacillus is a genus of gram-positive facultative anaerobic lactic acid bacteria. The lactic acid bacid bacteria are so named because most of its member?s convert lactose and other sugars to lactic acid. They are common and usually benign. Many species are prominent in decaying plant material. The production of lactic acid makes its environment acidic which inhibits the growth of some harmful bacteria. Three types of media are generally used in microbiological assays namely maintenance media, inoculum /cultivation media and the test assay media.
Micro Vitamin Test Agar is used for carrying stock cultures of Lactobacilli and other test organisms used in microbiological assays. This media can also be used for routine cultivation of Lactobacilli in microbiological assays of vitamins and in inoculum preparation for assays. Stock cultures are prepared by stab inoculation in triplicates. One is used for preparation of stock cultures while others are used for inoculum preparation for assays. Transfer of cultures should be made at weekly or biweekly intervals.
Suspend a 16-24 hours? culture of Lactobacilli from Micro Vitamin Test Culture Agar into Micro Vitamin Test Inoculum Broth. After an incubation at 35-37?C for 18-24 hours, centrifuge the culture and decant the supernatant. Re-suspend the centrifuged cells in 10 ml of sterile saline suspension. Repeat the washing two more times. Dilute the washed cell suspension with basal assay medium or as desired to obtain the required density of cells. For procedure of Vitamin Assay, refer standard references.

for cultivation and maintenance of stock cultures of used in microbiological assays of vitamins