MINERAL MODIFIED GLUTAMATE BROTH BASE (as per ISO 16649)

Minerals Modified Glutamate Broth Base is recommended by ISO 16649-3 for enumeration of E.coli using the most probable number Method (MPN). This medium is also an alternative broth used for the presumptive identification of coliforms in water.
ISO 16649-3 recommend to inoculate three tubes of a single strength and three tubes of a double strength medium. The tubes of double- and single-strength medium are inoculated at 37 ?C for 24 h. The tubes are examined for acid production, indicating lactose fermentation. Each tube of selective enrichment medium showing acid production is subcultured to TBX Chromogenic Agar (TM 1339).

for enumeration of coliform bacteria in water and wastewater samples