MINIMAL BROTH, DAVIS W/O DEXTROSE

Minimal media can be supplemented with the desired additives to study nutritional characters of the nutritional mutants. Minimal media are the formulations of Davis as described by Lederberg.
A cell suspension of wild type Escherichia coli is irradiated and cultured on Minimal Agar and incubated at 35?C for 24 hours. The isolated colonies are cultured in tubes of Minimal Broth Davis and Minimal Broth Davis without Dextrose. After 24 hours? incubation at 35?C growth in the Minimal Broth, Davis and absence of growth in the Minimal Broth Davis without Dextrose indicates a mutant.

for isolation and characterization of nutritional mutants of Escherichia coli