MOTILITY NITRATE MEDIUM, BUFFERED

Clostridium perfringens food poisoning is one of the most common type of human foodborne illness. The foods usually involved are cooked meat or poultry products containing large number of viable cells. Clostridium perfringens is a gram-positive, rod shaped anaerobic, spore-forming bacteria that produces enterotoxin. This toxin if ingested, can cause food poisoning. Motility Nitrate Medium, buffered formulated in accordance with FDA and APHA, is recommended for the detection of C. perfringens on the basis of motility and nitrate test.
In the nitrate reduction test, a pink to red color develops after addition of the reagents if nitrite is present. Colour development indicates that nitrate reduction has occurred in the tube. Some organisms further reduce nitrite to ammonia that can be detected by the addition of a small amount of zinc dust to the tubes exhibiting no colour. A pink colour in this part of the test indicates no nitrate reduction. A colourless reaction indicates that nitrates have been completely reduced. Inoculate 2 grams of food sample in 15 to 20 ml of Chopped Liver Broth or Tryptone Glucose Yeast Extract Broth. After an incubation at 35-37?C for 20-24 hours, isolate on Perfringens Agar Base (TSC/SFP Agar Base).
Presumptive C. perfringens colonies are confirmed biochemically by inoculating into Motility Nitrate Medium, buffered to detect motility and nitrate reduction.

for isolation and detection of Clostridium perfringens on the basis of motility and nitrate test