MUELLER HINTON AGAR (VEG.)

These media are prepared by completely replacing animal based peptones with vegetable peptones. Mueller Hinton Veg Agar is the modification of Mueller Hinton Agar which is recommended for the diffusion of antimicrobial agents impregnated on paper disc through an agar gel as described in CLSI Approved Standard. Mueller Hinton Veg Broth is used for determining Minimal Inhibitory Concentration (MIC) of antimicrobials for aerobic bacteria. Kirby-Bauer et al recommended this medium for performing antibiotic susceptibility tests using a single disc of high concentration.
A standardized suspension of the organisms is swabbed over the entire surface of the agar medium. Paper discs impregnated with certain amount of specific antibiotics are placed on the surface of the medium. The plates are incubated and the zones of inhibition around each disc are measured. It is then determined whether the organism is susceptible, intermediate or resistant to an agent by comparing the zone-sizes to standard zone-sizes. Different factors influence the disc diffusion susceptibility tests as, inoculum concentration, agar depth, disc potency, medium pH and beta-lactamase production by test organisms.

for cultivation of Neisseria spp. & for determination of susceptibility of microorganisms to antimicrobial agents isolated from clinical samples