MUELLER HINTON BROTH

Mueller Hinton Broth is a stable serum-free bacteriological media that supported the growth of two otherwise very fastidious bacteria, the meningococcus and the gonococcus. Later it was improved by supplementing cations to enhance Pseudomonas recovery and for propagating the vast majority of pathogenic bacteria encountered in clinical practice. In the beginning of the antibiotic era, the versatility of Mueller Hinton Broth and its agar was a perfect match, allowing a standardized, consistent medium for broth dilution or disk diffusion testing of antibiotic potency, defined most frequently in terms of minimum inhibitory concentration (MIC). In recent decades, MHB has been endorsed by the Clinical and Laboratory Standards Institute (CLSI), the global nonprofit organization that ensures quality in healthcare testing, as the appropriate media for routine bacterial antibiotic susceptibility determination, with updated cutoff standards for designating resistant and susceptible strains.

for testing the susceptibility of microorganisms to sulphonamides by tube dilution method