MUG BRILLIANT GREEN BILE BROTH

Brilliant Green Bile Broth is one of the most widely used medium for the detection of coliform bacteria in water, wastewater, foods, and milk and dairy products. This medium is formulated as per APHA for the presumptive identification and confirmation of coliform bacteria.
The fluorogenic compound, MUG (4- Methylumbelliferyl-?-D-glucuronide) in the medium permits the rapid detection of E. coli which produces a blue fluorescence when hydrolyzed by the enzyme ?-glucuronidase and is observed using a long-wave UV light source. During examination of water samples, growth from presumptive positive tubes showing gas in Lactose Broth or Lauryl Tryptose Broth is inoculated in Brilliant Green Bile Broth 2%. Gas formation within 48 ? 2 hours confirms the presumptive test.

for detection of Escherichia coli in water and food by a ?uorogenic method