MUG EC BROTH

Escherichia coli is a member of faecal coliform group of bacteria. It is a member of the indigenous faecal flora of warm- blooded animals. E. coli is considered a specific indicator of faecal contamination and the possible presence of enteric pathogens. EC Broth was devised by Hajna and Perry and further modified by addition of the fluorogenic compound MUG. MUG EC Broth is also recommended by APHA for the analysis of drinking water, surface and ground water and waste-water for the presence of E. coli. MUG permits rapid detection of E. coli when medium is observed for fluorescence using UV Light. MUG also detects anaerogenic strains which may not be detected in conventional procedure. MUG is hydrolyzed by the enzyme ??glucuronidase possessed by E. coli to yield a fluorescent end product 4-Methylumbelliferone. Large number of Proteus vulgaris if present, may suppress gas production of E. coli, however fluorescence permits detection of E. coli in pure or mixed cultures within 4 to 24 hours

for detection of Escherichia coli in water and food by ?uorogenic method