MYCOPLASMA BROTH BASE W/O CV (PPLO BROTH BASE W/O CV)

PPLO Broth without crystal violet is prepared according to the formula described by Morton and Lecce. Crystal violet is omitted from this formula due to its inhibitory action on some Mycolasma. It has been used for the cultivation of Mycoplasma for research studies. For the cultivation of Mycoplasma, the medium ingredients and all the supplements should be free of any toxic substances even in small amounts. Many Mycoplasma require serum for their good growth and also presence of antibiotic is necessary to prevent the growth of contaminating organisms. Mostly the Mycoplasma species are aerobic or facultatively anaerobic but some are microaerophilic. Few are anaerobic saprophytic Mycoplasma which grow best at 22-35?C while pathogenic strains grow at 35?C.

for enrichment of Mycoplasma species (PPLO) from clinical samples and mixed cultures