MYCOPLASMA SYNOVIAE MEDIUM BASE

Genus Mycoplasma belongs to the class Mollicutes characterized by absence of cell wall, small genome and low G + C content, and were first recognized from a case of pleropneumonia in a cow. The organism was designated ?pleuropneumonia-like organism?, or PPLO. For the cultivation of Mycoplasma, the medium ingredients and all the supplements should be free of any toxic substances even in small amounts. Calf brain infusion from, beef heart infusion from and proteose peptone provide nitrogen, vitamins, amino acids and carbon sources. Sodium chloride maintains the osmotic balance. Many Mycoplasma require serum for their good growth and also presence of antibiotic is necessary to prevent the growth of contaminating organisms. Mostly the Mycoplasma species are aerobic or facultatively anaerobic but some are microaerophilic. Few are anaerobic saprophytic Mycoplasma which grow best at 22-35?C while pathogenic strains grow at 35?C.

for cultivation of avian strains of Mycoplasma