NUTRIENT AGAR NO. 2 (as per BIS)

Nutrient Agar is a general purpose medium used for the examination of water and dairy products according to Standard Methods for the Examination of Water and Waste water and Dairy Products. Nutrient Agar No. 2 is used in microbiological analysis of water by Czech Standards. It can also be used for cultivating several less fastidious microorganisms. It is recommended by BIS for the cultivation of Escherichia coli, Staphylococcus aureus and Vibrio from food samples. Nutrient Agar with 0.8% sodium chloride and pH 6.0 is used for cultivation of bacteria requiring slightly acidic conditions. Nutrient media may be used as enriched media by addition of 10% blood or other biological fluids like ascetic fluid, serum etc.