PLESIOMONAS DIFFERENTIAL AGAR (INOSITOL BRILLIANT GREEN BILE AGAR)

Plesiomonas differential Agar (Inositol Brilliant Green Bile Agar) is a medium described by Schubert and is recommended for selective isolation of P.shigelloides (an opportunistic pathogen) and Aeromonas species from faeces and other foodstuffs. Several media and methods have been designed to selectively isolate P.shigelloides. Strains of P.shigelloides grow in the presence of brilliant green and are also resistant to bile salts that are usually incorporated in media to inhibit gram-positive bacteria. Most bacterial species do not ferment meso-inositol, but almost all strains of P.shigelloides ferment this to naturally occurring cyclic polyhydroxyl alcohol. Schubert took advantage of the three properties as discussed above and designed Inositol Brilliant Green Bile Salts Agar. It is a differential medium for inositol utilizers and non-utilizers.

for selective isolation of Plesiomonas shigelloides and Aeromonas species from foods