R-2A BROTH

R-2A Broth is similar to R-2A Agar except agar. Total count recommended for the bacterial examination of potable waters gives an estimate of the aerobic and facultatively anaerobic bacteria, which grow best at 35?C in a rich medium. R-2A Broth enables better recovery of these bacteria from treated waters under different incubation conditions. Many bacteria from natural waters, which contain limited nutrients at ambient temperature, grow best on the media with less nutrient levels. They grow better at the temperatures below the routine laboratory incubation temperatures of 35 to 37?C.
The total bacterial count of drinking water is determined by plate count on a nutritionally rich medium. However, all organisms present are not able to grow on them, either because they are slow growers or because they can?t grow on that media. For this reason, a nutritionally reduced medium was described. R-2A Agar is a modification of this medium.

for cultivation and maintenance of heterotrophic bacteria from potable waters