RAPID COLIFORM BROTH

Rapid Coliform Broth is a modification of LMX Broth described by Manafi and Kneifel. This medium is useful for the detection and confirmation of Escherichia coli and total coliforms in water samples on the basis of chromogenic and fluorognic substrates. The fluorogenic substrate is split by enzyme ?-D-glucuronidase specifically found in Escherichia coli. The reaction is indicated by the development of a blue fluorescence under UV light. The presence of total coliforms is indicated by bluegreen colourations due to the cleavage of the chromogenic substrate. To confirm presence of E.coli overlay the medium with Kovacs reagent. The layer turns red within 2 minutes in case of positive reaction.

for detection and con?rmation of E.coli and other coliforms on the basis enzyme substrate reaction from water by using chromogenic and ?uorogenic substrates