REINFORCED CLOSTRIDIAL BROTH (as per USP/BP/JP/EP)

Reinforced Medium for Clostridia was formulated by Hirsch and Grinsted and is in accordance with the microbial limit testing by harmonized methodology of USP/EP/BP/JP/IP. It is recommended for sterility checking of non-sterile products, nutritional and dietary supplements. It can be used to initiate growth from small inocula and to obtain the highest viable count of clostridia. Barnes and Ingram used the broth medium for diluting an inoculum of vegetative cells of Clostridium perfringens. It can be used in studies of spore forming anaerobes, especially Clostridium butyricum in cheese, for enumeration of Clostridia in tube dilution counts or for preparation of plates for isolation. Other spore forming anaerobes, Streptococci and Lactobacilli also grow in these media.