REINFORCED CLOSTRIDIAL BROTH (as per USP/BP/JP/EP)

Reinforced Clostridial Broth is an enriched, non-selective medium formulated by Hirsch and Frinsted. This medium was developed for the isolation of spore-forming anaerobes, especially Clostridium spp. It conforms to the harmonized USP/EP/JP/BP/IP requirements. This medium can be used for diluting an inoculum of vegetative cells of Clostridium perfringens as suggested by Barnes and Ingram. It can also be used in studies of spore forming anaerobes, especially Clostridium butyricum in cheese or for enumeration of Clostridium species in tube dilution counts. Other spore forming anaerobes, Streptococci and Lactobacilli also grow in these media.

for isolation, cultivation and enumeration of Clostridia species, highly nutritive for Clostridium sporogenes and other anaerobes