ROSE BENGAL AGAR W/ CHLORTETRACYCLINE

Rose Bengal Agar Base is a modification of the Rose Bengal Chlortetracycline Agar formula of Jarvis. Of the antibiotics most frequently employed in media of neutral pH, Chloramphenicol is recommended because of its heat stability and broad antibacterial spectrum. Rose Bengal Agar media are recommended in standard methods for the enumeration of yeasts and molds from food stuffs and water.
The use of media with an acid pH that selectively inhibits the growth of bacteria and thereby promotes the growth of fungi has been widely employed. A number of investigators have reported, however, that acidified media may actually inhibit fungal growth, fail to completely inhibit bacterial growth and have little effect in restricting the size of mould colonies. Smith and Dawson used rose bengal in a neutral pH medium for the selective isolation of fungi from soil samples. Chloramphenicol, streptomycin, oxytetracycline and chlortetracycline have been used for the improved, selective isolation and enumeration of yeasts and molds from soil, sewage and foodstuffs.

for the selective isolation and enumeration of yeasts and moulds