SKIM MILK AGAR

SM Agar is used for the demonstration of coagulation and proteolysis of casein. The medium is recommended by APHA for cultivation and enumeration of microorganisms encountered in dairy industry. Addition of SM powder to any nutrient-rich medium creates favorable conditions for growth of organisms, which are encountered in milk. The number of bacteria isolated thus is more than the number of organisms isolated on a regular medium. Proteolytic bacteria hydrolyze casein to form soluble nitrogenous compounds indicated as clear zone surrounding the colonies. More clear zones are seen on milk agar if, the bacteria produce acid from fermentable carbohydrates in the medium.

for cultivation and enumeration of bacteria encountered in dairy industry