SOYABEAN BILE BROTH BASE

Soyabean Bile Broth Base is formulated as recommended by FDA for the enrichment and isolation of E. coli O157:H7.
Whenever low levels of E. coli O157:H7 are suspected, the food is enriched in Soyabean Bile Broth and further plated on selective medium as Sorbitol MacConkey Agar or Hemorrhagic coli (HC) Agar for isolation and identification.
Blend 25 gm food sample to be tested in 224 ml Soyabean Bile Broth and incubate with shaking (about 100 rpm) at 37?C for 18-24 hours. Prepare dilution of the enrichment culture with phosphate buffer and spread 0.1 ml of each dilution on HC Agar or Sorbitol MacConkey Agar plates and incubate at 43?C for 24 hours.

for enrichment and isolation of Escherichia coli 0157:H7 from foods