T.A.T. BROTH WITH TWEEN 20 (as per USP)

T.A.T. Broth is prepared according to the formula recommended by United States Food and Drug Administration and United States Pharmacopoeia for enrichment and further isolation and cultivation of gram-negative bacteria in cosmetics, tropical drugs and sterility testing of viscous or gelatinous substances. Prepare decimal dilutions of the sample to be tested from 10-1 to 10-6. Inoculate 1 gram (1 ml) sample and 1 ml of each dilution into 40 ml of T.A.T. Broth. After incubation subculture the growth on MacConkey Agar and TSI Agar.

for sterillity testing of highly viscous or gelatinous substances in Cosmetic and Pharma