TERRIFIC BROTH (TARTOFF – HOBBS BROTH)

Tartoff Hobbs developed the Terrific Broth Medium for cultivation of recombinant Escherichia coli strains. These strains have extended growth phase when cultivated in this medium. Tartoff-Hobbs Medium supports high cellular density and mass and maintains the growth in the logarithmic phase for a long time. Due to this fact, it provides greater yields of recombinant proteins and plasmid DNA. Often, Tartoff-Hobbs (Terrific) Broth substitutes Luria Bertani Broth, to get enhanced yields of plasmid DNA and recombinant proteins. The procedures for inoculation, incubation and generation of recombinant strains are detailed by Sambrook et al.

a bu?ered enriched medium for propagation of recombinant E. coli