TEEPOL BROTH (DOUBLE PACK)

Faecal coliform bacteria are a group of bacteria passed through faecal excrement of humans, livestock and wild life. They are used as indicators of faecal pollution in water such as waste waters, effluents, rivers, marine environments, recreational waters and raw sources of drinking water supplies. The use of teepol in place of bile salts was previously recommended by Jameson and Emberley. Burman showed that if a preliminary incubation is carried out at lower temperature resuscitation is not required. Non-chlorinated organisms benefit from 4 hours? incubation at 30?C but chlorinated organisms require 6 hours incubation at 25?C. The coliform and Escherichia coli count are made on separate volumes of water. The water samples are filtered through membrane filter and this filter is placed face upwards on an absorbent pad saturated with Teepol Broth. The yellow colonies formed are further identified.

for selective isolation and identification of enteric, lactose fermenting bacteria