TETRATHIONATE BRILLIANT GREEN BILE BROTH

Salmonella are gram-negative, facultatively anaerobic, non-sporulating, non-motile rods in the family Enterobacteriaceae. They are widely distributed in animals affecting mainly the stomach and the intestines. These organisms are difficult to differentiate biochemically from Escherichia coli. Tetrathionate Broth was originally described by Mueller and later modified by Kauffman. Tetrathionate Brilliant Green Bile Broth is used as an enrichment medium for Salmonella. Enrichment broth is usually recommended to facilitate the recovery of small numbers of Salmonella species. Tetrathionate Brilliant Green Bile Broth is also mentioned in I.P. for isolation and identification of Salmonella species from foods, water and other materials of sanitary importance. After incubation, streak the culture from Tetrathionate Brilliant Green Bile Broth onto differential medium for isolation and identification. Tetrathionate Brilliant Green Bile Broth is not suitable for growth of Salmonella Typhi and Salmonella Paratyphi.

for isolation and identi?cation of Salmonellae